Fig 1: CNBP was upregulated in MDR cells. The influence of CNBP on cell proliferation and apoptosis in HCC cells. The level of CNBP assessed via Western blot (a) and (b), cell proliferation measured by CCK-8 assay (c); colony formation ability (d) and (e), and cell apoptosis analyzed by flow cytometry (f) and TUNEL assay (g), (h) in the study groups. ***p < 0.001 vs. control group; #, p < 0.05, ##, p < 0.01 and ###, p < 0.001 vs. SOR group; $, p < 0.05, $$, p < 0.01 and $$$, p < 0.001 vs. SOR + NC group.
Fig 2: CNBP was confirmed as the binding protein of circFMN2. CNBP level was measured via Western blot (a), (b) lanes in panel A were corresponding to columns in panel B, Western blot analysis of CNBP level after RNA pull-down assay (c), circFMN2 level detected by RT-qPCR following RNA immunoprecipitation assay (d), The deletion fragments, sense strand and anti-sense strand of circFMN2 in deletion-mapping analysis (e), Western analysis of CNBP level following RNA pull-down assay with different circFMN2 constructs in deletion-mapping analysis (f), and ubiquitination level detected by Western blot assay (g). ***p < 0.001 vs. control group; ###, p < 0.001 vs. circFMN2-NC group; &&&, p < 0.001 vs. MDR group; $$$, p < 0.001 vs. MDR + siRNA-NC group.
Supplier Page from Abcam for Anti-CNBP antibody